Analysis of the mutagenic activity of drinking water samples and soil water extracts from storage sites that are not disposed of and banned for use by pesticides on human blood lymphocyte cultures

Abstract

The article presents the results of a study of drinking water samples and soil water extracts from three different villages in the Almaty region, located near the storage of unused, obsolete, and prohibited pesticides. The study has been carried out by adding 5 and 10% (concentration from culture medium) aqueous and water soil extracts to different cultures of lymphocytes from different settlements in the Almaty region (Belbulak, Amangeldy and Basshi - control). The addition of 5% of drinking water and soil extracts to the lymphocyte cultures has revealed only a tendency to increase the frequency of chromosomal aberrations in the villages of Belbulak and Amangeldy. An analysis of the state of the studied 10% water samples and soil extracts from the three examined villages in the Almaty region has shown that the addition of lymphocytes to the culture medium revealed statistically significant mutagenicity of drinking water for the population in the villages of Amangeldy and Belbulak. Consequently, a 2-fold and 1.5-fold increase in the frequency of chromosomal abnormalities in Belbulak settlement in lymphocyte culture for Basshi settlement (Altyn-Emel National Park) indicates a generally low quality of drinking water, which can adversely affect the health of people living in this rural area of Almaty region. Evaluation of genetic status of rodents caught in such monitoring places as Belbulak, Amangeldy, Kyzylkairat, Enbekshi, near the storages of old unutilized pesticides, indicates a significant increase in the frequency of disorders of genetic apparatus of the studied animals. There has been revealed a statistically significant correlation between both the test systems used on rodents and the frequency of chromosomal aberrations in human lymphocyte cultures during the mutagenicity analysis of soil samples from the studied settlements.